AKTA Pure FPLC Protein Purification System

AKTA Pure FPLC Protein Purification System

The AKTA Pure FPLC Protein Purification System is a high-performance platform designed for precise, reliable, and reproducible protein purification in research and development laboratories. Engineered for flexibility and efficiency, this system combines advanced chromatography technologies with intuitive software control, enabling scientists to perform complex purification protocols with ease.

With modular components including pumps, sample injectors, detectors, and fraction collectors, the AKTA Pure system delivers exceptional control over flow rates, gradients, and separation conditions. Its precise and stable operation ensures high-resolution purification, minimizing sample loss and preserving protein integrity.

The system user-friendly interface, combined with the UNICORN software, allows seamless method development, process optimization, and automated workflows. Ideal for applications in biopharmaceutical development, structural biology, and protein characterization, the AKTA Pure FPLC system supports a wide range of chromatography techniques, including ion exchange, affinity, size exclusion, and hydrophobic interaction chromatography.

Built for reproducibility, versatility, and efficiency, the AKTA Pure FPLC Protein Purification System provides researchers with a reliable solution for high-quality protein purification, enabling consistent results and accelerating scientific discovery.

Key Features

High-Performance FPLC – Ensures precise and reproducible protein purification with minimal sample loss.

Modular Design – Configurable pumps, detectors, sample injectors, and fraction collectors to suit diverse purification workflows.

UNICORN Software Integration – User-friendly interface for method development, process control, and automated protocols.

Flexible Chromatography Techniques – Supports ion exchange, affinity, size exclusion, and hydrophobic interaction chromatography.

Precise Flow Control – High-resolution separation with stable, adjustable flow rates.

Automated Fraction Collection – Efficient sample handling and reproducible collection for downstream analysis.

Real-Time Monitoring – Integrated detectors allow continuous monitoring of protein elution and sample quality.

Scalable Workflow – Suitable for both analytical and preparative scale purification.

High Reproducibility – Consistent results across multiple runs, ideal for research and biopharmaceutical development.

Robust and Reliable – Built for long-term laboratory use with minimal maintenance.

Technical Specifications

Pump

Type: Dual-piston, high-precision pump

Flow Rate Range: 0.001 – 25 mL/min

Flow Accuracy: ±1 %

Flow Precision: ≤0.1 % RSD

Maximum Pressure: Up to 30 MPa (300 bar)

Gradient Accuracy: ±1 %

Sample Injection

Injection Volume Range: 5 µL – 5 mL (depending on configuration)

Injection Modes: Manual or automated sample injection

Sample Loop Options: Variable volumes to suit analytical or preparative workflows

Column Compartment

Temperature Range: Ambient – 70 °C (depending on accessory module)

Temperature Stability: ±0.1 °C

Column Sizes Supported: 0.1 – 120 mL (analytical to preparative columns)

Detection

UV/VIS Detector: 200 – 800 nm wavelength range

Flow Cell Volume: 10 – 100 µL (depending on detector configuration)

Optional Detectors: Conductivity, pH, fluorescence detectors

Fraction Collector

Capacity: Up to 60 tubes standard; configurable for larger collection volumes

Fraction Sizes: Adjustable, automated collection by volume or time

Automation: Compatible with UNICORN software for scheduled fraction collection

Software & Control

Control Software: UNICORN 7 or higher

Features: Method development, gradient programming, real-time monitoring, automated purification protocols

Data Export: Compatible with multiple formats for downstream analysis

General & System Features

Dimensions (L×W×H): Approx. 600 × 400 × 400 mm (system dependent)

Weight: ~40 kg (system dependent)

Power Requirements: 100–240 V, 50/60 Hz

Applications: Protein purification, antibody purification, structural biology, biopharmaceutical R&D

Scalability: Supports both analytical and preparative purification workflows